• Product NameVimentin Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total vimentin protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from Internal of human vimentin.
• Target NameVimentin
• Alternative Names    VIME; vimentin;
• Accession No. Swiss-Prot: P08670
  NCBI Gene ID: 7431
• UniprotP08670
• Gene ID7431;
• SDS-PAGE MW 53kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

WB Isaacs, J. Biol. Chem., Oct 1989; 264: 17953 - 17960.
Patric Turowski, Mol. Biol. Cell, Jun 1999; 10: 1997.
Melanie L. Styers, J. Cell Sci., Sep 2006; 119: 3643 - 3654.
Qing-Fen Li, J. Biol. Chem., Nov 2006; 281: 34716 - 34724.

• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Vimentin antibody #33541.
• Western blot analysis of extracts from HepG2 cells, treated with Adriamycin (0.5uM, 5hours), using Vimentin antibody #33541.
• Western blot analysis of extracts from 293 cells (Lane 2), using Vimentin antiobdy #33541. The lane on the left is treated with synthesized peptide.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting