• Product NameJunD(Phospho-Ser255) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of JunD only when phosphorylated at serine 255.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 255 (G-E-S(p)-P-P) derived from Human JunD.
• Target NameJunD
• ModificationPhospho
• Alternative Names    AP-1
• Accession No. Swiss-Prot: P17535
  NCBI Protein: NP_005345.3
• UniprotP17535
• Gene ID3727;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 38kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  

Binds an AP-1 site and upon cotransfection stimulates the activity of a promoter that bears an AP-1 site.

Beausoleil S A, et al. (2004) Proc Natl Acad Sci U S A. 101(33): 12130-12135.

• Western blot analysis of extracts from 293 cells using JunD(Phospho-Ser255) Antibody #11028(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
• Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JunD(Phospho-Ser255) Antibody #11028(left) or the same antibody preincubated with blocking peptide(right).

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting