• Product NamePKCb(Phospho-Thr641) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of PKCb only when phosphorylated at threonine 641.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 641 (E-L-T(p)-P-T) derived from Human PKCb
• Target NamePKCb
• ModificationPhospho
• Alternative Names    PKCB; PRKCB1; PRKCB2
• Accession No. Swiss-Prot: P05771
  NCBI Protein: NP_002729.2
• UniprotP05771
• Gene ID5579;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 82kd
  Western blotting: 1:500~1:1000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:200

Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.

Zhang Y, et al. (2006) Mol Cell Biol ; 26: 6748-6761
Castoria G, et al. (2004) Mol Cell Biol ; 24: 7643-7653
Marcil J, et al. (1999) Biochem J ; 337:185-192
Bornancin F, et al. (1996) Curr Biol ; 6:1114-1123.

• Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using PKCb(phospho-Thr641) antibody(#11172).
• Immunofluorescence staining of methanol-fixed MCF7 cells using PKCb(phospho-Thr641) antibody(#11172, Red).
• Western blot analysis of extracts from JK cells untreated or treated with PMA using PKCβ (phospho-Thr641) antibody #11172.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting