• Product NameTrkB(Ab-705) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total TrkB protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.703~707 (T-D-Y -Y-R) derived from Human TrkB.
• Target NameTrkB
• Alternative Names    TRKB; trk-B; GP145-TrkB
• Accession No. Swiss-Prot: Q16620
  NCBI Protein: NP_001007098.1
• UniprotQ16620
• Gene ID4915;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 145kd
  Western blotting: 1:500~1:1000
  Immunofluorescence: 1:100~1:200

Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.

Woronowicz A, et al. Glycobiology. 2007 Jan;17(1):10-24. Mojsilovic-Petrovic J, et al. J Neurosci. 2006 Sep 6;26(36):9250-63. Lewis MA, et al. Mol Pharmacol. 2006 Apr;69(4):1396-404. Cai D, et al. Physiol Genomics. 2006 Feb 14;24(3):191-7.

• Western blot analysis of extract from C6 cell and using TrkB(Ab-705) Antibody #21328.
• Immunofluorescence staining of methanol-fixed NCI-h446 cells showing cytoplasmic staining using TrkB (Ab-705) Antibody #21328.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting