• Product NameDNA Polymerase θ Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC IF
• Species ReactivityMouse
• SpecificityThe antibody detects endogenous levels of total DNA Polymerase θ protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human DNA Polymerase θ.
• Target NameDNA Polymerase θ
• Alternative Names    DNA polymerase theta; EC 2.7.7.7; DNA polymerase eta; POLQ; POLH
• Accession No. Swiss-Prot: O75417
  NCBI Gene ID: 10721
• UniprotO75417
• Gene ID10721;
• SDS-PAGE MW 200kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• IF：1:50-200
  Immunofluorescence：1:50-200

Has a DNA polymerase activity on nicked double-stranded DNA and on a singly primed DNA template. The enzyme activity is resistant to aphidicolin, and inhibited by dideoxynucleotides. Exhibites a single-stranded DNA-dependent ATPase activity. Could be involved in the repair of interstrand cross-links.

Sharief F.S., Genomics 59:90-96(1999).
Harris P.V., Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.

• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using DNA Polymerase θ antibody #33611.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting