• Product NameeIF2b(phospho-Ser2) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of eIF2b only when phosphorylated at serine 2.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 2 (M-S(p)-G-D-E) derived from Human eIF2β.
• Target NameeIF2b
• ModificationPhospho
• Alternative Names    EIF2S2; EIF2; EIF2B
• Accession No. Swiss-Prot: P20042
  NCBI Protein: NP_003899.2
• UniprotP20042
• Gene ID8894;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 38kd
  Western blotting: 1:500~1:1000

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Structure of the beta subunit of translational initiation factor eIF-2. The DNA sequence and comparative analysis of human chromosome 20. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

• Western blot analysis of extracts from C2C12 cells untreated or treated with PMA using eIF2b(phospho-Ser2) Antibody #11512.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting