• Product NameMBD3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total MBD3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human MBD3.
• Target NameMBD3
• Alternative Names    Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3; MBD3;
• Accession No. Swiss-Prot: O95983
  NCBI Gene ID: 53615
• UniprotO95983
• Gene ID53615;
• SDS-PAGE MW 33kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (. Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (). Recruits histone deacetylases and DNA methyltransferases.

Hendrich B., Mol. Cell. Biol. 18:6538-6547(1998).
Grimwood J., Nature 428:529-535(2004).
Tatematsu K., Genes Cells 5:677-688(2000).

• Western blot analysis of extracts from Jurkat cells, using MBD3 antibody #33640.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting