• Product NameEphrin-B2(Phospho-Tyr316) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of Ephrin-B2 only when phosphorylated at tyrosine 316.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 316 (P-V-Y(p)-I-V) derived from Human Ephrin-B2
• Target NameEphrin-B2
• ModificationPhospho
• Alternative Names    HTKL; EPLG5; Htk-L; LERK5;
• Accession No. Swiss-Prot: P52799
  NCBI Protein: NP_004084.1
• UniprotP52799
• Gene ID1948;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 37kd
  Western blotting: 1:500~1:1000
  

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

Chrencik JE,et al.( 2006) J Biol Chem;281(38):28185-28192.
Kertesz N, et al.( 2006) Blood;107(6):2330-2338.
Noren NK, et al.( 2004) Proc Natl Acad Sci USA;101(15):5583-5588

• Western blot analysis of extracts from HT29 cells, untreated or treated with UV using Ephrin-B2(Phospho-Tyr316) Antibody #11188.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting