• Product NameM-CK Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total M-CK protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human M-CK.
• Target NameM-CK
• Alternative Names    CKM; CKMM.; EC 2.7.3.2; KCRM; creatine kinase
• Accession No. Swiss-Prot: P06732
  NCBI Gene ID: 1158
• UniprotP06732
• Gene ID1158;
• SDS-PAGE MW 43kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Grimwood J., Nature 428:529-535(2004).
Hamburg R.J., J. Biol. Chem. 265:6403-6409(1990).
Tang L., Acta Crystallogr. D 55:669-670(1999).

• Western blot analysis of extracts from HUVEC cells, HepG2 cells, Jurkat cells and HeLa cells, using M-CK antibody #33654.
• Immunohistochemistry analysis of paraffin-embedded human heart tissue using M-CK antibody #33654.
• Immunofluorescence analysis of HepG2 cells, using M-CK antibody #33654.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting