• Product NameAdrenergic Receptor α-2A Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total Adrenergic Receptor α-2A protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Adrenergic Receptor α-2A.
• Target NameAdrenergic Receptor α-2A
• Alternative Names    A2AA; ADA2A; ADRA2R; ADRAR; Alpha-2A adrenergic receptor
• Accession No. Swiss-Prot: P08913
  NCBI Gene ID: 150
• UniprotP08913
• Gene ID150;
• SDS-PAGE MW 48kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is oxymetazoline > clonidine > epinephrine > norepinephrine > phenylephrine > dopamine > p-synephrine > p-tyramine > serotonin = p-octopamine. For antagonists, the rank order is yohimbine > phentolamine = mianserine > chlorpromazine = spiperone = prazosin > propanolol > alprenolol = pindolol.

Kobilka B.K., Science 238:650-656(1987).
Fraser C.M., J. Biol. Chem. 264:11754-11761(1989).
Guyer C.A., J. Biol. Chem. 265:17307-17317(1990).

• Western blot analysis of extracts from LOVO cells and Jurkat cells, using Adrenergic Receptor α-2A antibody #33660.
• Immunohistochemistry analysis of paraffin-embedded human testis tissue using Adrenergic Receptor α-2A antibody #33660.
• Immunofluorescence analysis of NIH/3T3 cells, using Adrenergic Receptor α-2A antibody #33660.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting