• Product NameFAK(Ab-397) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total FAK protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 395~399 (D-D-Y-A-E) derived from Human FAK.
• Target NameFAK
• Alternative Names    FADK; PTK2; FAK1; FRNK; PTK2
• Accession No. Swiss-Prot: Q05397
  NCBI Protein: NP_005598.3
• UniprotQ05397
• Gene ID5747;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 125kd
  Western blotting: 1:500~1:1000

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.

von Sengbusch A, et al. (2005) Am J Pathol ; 166(2): 585-596
Fornaro M, et al. (2000) Mol Biol Cell ; 11(7): 2235-2249
Zhang X, et al. (1999) Proc Natl Acad Sci U S A; 96(16): 9021-9026.

• Western blot analysis of extract from HepG2, 293, C6, HUVEC and HT29 cells using FAK(Ab-397) Antibody #21208

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting