• Product NameSP1(Phospho-Thr739) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of SP1 only when phosphorylated at threonine 739.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 739 (T-A-T(p)-P-S) derived from human SP1
• Target NameSP1
• ModificationPhospho
• Alternative Names    TSFP1; Sp1 transcription factor;
• Accession No. Swiss-Prot: P08047
  NCBI Protein: NP_612482.2
• UniprotP08047
• Gene ID6667;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 90kd
  Western blotting: 1:500~1:1000
  

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-a G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression.

Thiesen HJ,et al.(1990)Nucleic Acids Res.18(11):3203-9.
Zutter MM,et al.(1997) Blood.90(2):678-89.
Lou Z,et al.(2005)Cancer Res.65(3):1007-17

• Western blot analysis of extracts from 3T3 cells treated with starvation using SP1(Phospho-Thr739) Antibody #11241.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting