• Product NameHistone H2AX Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Histone H2AX protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Histone H2AX.
• Target NameHistone H2AX
• Alternative Names    H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X
• Accession No. Swiss-Prot: P16104
  NCBI Gene ID: 3014
• UniprotP16104
• Gene ID3014;
• SDS-PAGE MW 15kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

The MGC Project Team; Genome Res. 14:2121-2127(2004).
Rogakou E.P., J. Biol. Chem. 273:5858-5868(1998).
Rogakou E.P., J. Biol. Chem. 275:9390-9395(2000).

• Western blot analysis of extracts from HT-29 cells, using Histone H2AX antibody #33686.
• Immunofluorescence analysis of COS7 cells, using Histone H2AX antibody #33686.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting