• Product NameBRCA1(Phospho-Ser988) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsIF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of BRCA1 only when phosphorylated at serine 988.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 988 (I-K-S(p)-F-V) derived from Human BRCA1.
• Target NameBRCA1
• ModificationPhospho
• Alternative Names    RNF53
• Accession No. Swiss-Prot: P38398
  NCBI Protein: NP_009225.1
• UniprotP38398
• Gene ID672;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 220kd
  Immunofluorescence: 1:100~1:200

The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation

Lin SY, et al. (2004) Proc Natl Acad Sci U S A, 101(17): 6484-6489
Zhang J, et al. (2004) Mol Cell Biol; 24(2): 708-718
Beger C, et al. (2001) Proc Natl Acad Sci U S A; 98(1): 130-135
Gardner K, et al. (2001) Breast Cancer Res; 3(1): 11-13
Zheng L, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9587-9592

• Immunofluorescence staining of methanol-fixed Hela cells using BRCA1(Phospho-Ser988) Antibody #11237.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting