• Product NameATF3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total ATF3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ATF3.
• Target NameATF3
• Alternative Names    Cyclic AMP-dependent transcription factor ATF-3; Activating transcription factor 3; ATF3;
• Accession No. Swiss-Prot: P18847
  NCBI Gene ID: 467
• UniprotP18847
• Gene ID467;
• SDS-PAGE MW 21kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters.

Chen B.P.C., J. Biol. Chem. 269:15819-15826(1994).
Hai T., Genes Dev. 3:2083-2090(1989).
Hillman R.T., Genome Biol. 5:RESEARCH008.1-RESEARCH008.16(2004).

• Western blot analysis of extracts from RAW264.7 cells, using ATF3 antibody #33704.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting