• Product NameHNF4a(Phospho-Ser304) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of HNF4a only when phosphorylated at serine 304.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of serine 304 (L-R-S(p)-Q-V)derived from Human HNF4a.
• Target NameHNF4a
• ModificationPhospho
• Alternative Names    HNF4; TCF; MODY; HNF4A;
• Accession No. Swiss-Prot: P41235
  NCBI Protein: NP_000448.3
• UniprotP41235
• Gene ID3172;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 52kd
  Western blotting: 1:1000
  

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes,including hepatocyte nuclear factor 1 a, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants.

Kritis A.A,et al.Gene 173:275-280(1996)
Drewes T,et al.Mol. Cell. Biol. 16:925-931(1996)
Yamagata K,et al.Nature 384:458-460(1996)
Tanaka T,et al.Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases

• Western blot analysis of extracts from Hela cells untreated or treated with Anisomyclin using HNF4a(Phospho-Ser304) Antibody #11043.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting