• Product NameTFE3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total TFE3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human TFE3.
• Target NameTFE3
• Alternative Names    Transcription factor E3; TFE3;
• Accession No. Swiss-Prot: P19532
  NCBI Gene ID: 7030
• UniprotP19532
• Gene ID7030;
• SDS-PAGE MW 62kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.

Clark J., Oncogene 15:2233-2239(1997).
Sidhar S.K., Hum. Mol. Genet. 5:1333-1338(1996).
Beckmann H., Genes Dev. 4:167-179(1990).

• Western blot analysis of extracts from K562 cells and A549 cells, using TFE3 antibody #33708.
• Immunohistochemistry analysis of paraffin-embedded human brain tissue using TFE3 antibody #33708.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting