• Product NameTNF Receptor II Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF IHC ELISA
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total TNF Receptor II protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human TNF Receptor II.
• Target NameTNF Receptor II
• Alternative Names    Tumor necrosis factor receptor superfamily member 1B [Precursor]; Tumor necrosis factor receptor 2; TNF-R2; Tumor necrosis factor receptor type II; p75
• Accession No. Swiss-Prot: P20333
  NCBI Gene ID: 7133
• UniprotP20333
• Gene ID7133;
• SDS-PAGE MW 48kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

  IHC 1:50-1:200

  ELISA 1:20000-1:40000

Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

Smith C.A., Science 248:1019-1023(1990).
Kohno T., Proc. Natl. Acad. Sci. U.S.A. 87:8331-8335(1990).
Beltinger C.P., Genomics 35:94-100(1996).

• Western blot analysis of extracts from RAW264.7 cells, HT-29 cells and LOVO cells, using TNF Receptor II antibody #33710.
• Immunofluorescence analysis of HeLa cells, using TNF Receptor II antibody #33710.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting