• Product NameDGKA Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total DGKA protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human DGKA.
• Target NameDGKA
• Alternative Names    80 kDa diacylglycerol kinase; DAG kinase alpha; DAGK; DAGK1; DGK- alpha
• Accession No. Swiss-Prot: P23743
  NCBI Gene ID: 1606
• UniprotP23743
• Gene ID1606;
• SDS-PAGE MW 80kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.

Schaap D., FEBS Lett. 275:151-158(1990).
Hart T.C., Mamm. Genome 5:123-124(1994).
Hart T.C., Genomics 22:246-247(1994).

• Western blot analysis of extracts from Jurkat cells, using DGKA antibody #33723.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting