• Product NameSTAT2(phospho-Tyr690) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of Stat2 only when phosphorylated at Tyrosine 690.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of tyrosine 690 (R-K-Y(p)-L-K)derived from Human Stat2
• Target NameSTAT2
• ModificationPhospho
• Alternative Names    P113; ISGF-3; STAT113; MGC59816;
• Accession No. Swiss-Prot: P52630
  NCBI Protein: NP_005410.1
• UniprotP52630
• Gene ID6773;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 113kd
  Western blotting: 1:1000
  

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-a response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene.

Fu X.Y,et al.Proc. Natl. Acad. Sci. U.S.A. 89:7840-7843(1992)
Yan R,et al. Nucleic Acids Res. 23:459-463(1995)
Fu X.Y.Cell 70:323-335(1992)
Sugiyama T,et al.FEBS Lett. 381:191-194(1996)

• Western blot analysis of extracts from Hela cells untreated or treated with IFN-a using Stat2(phospho-Tyr690) Antibody #11536.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting