• Product NameCyclin E1(phospho-Thr395) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of Cyclin E1 only when phosphorylated at threonine 395.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around phosphorylation site of threonine 395 (L-L-T(p)-P-P)derived from Human Cyclin E1
• Target NameCyclin E1
• ModificationPhospho
• Alternative Names    CCNE; CCNE1;
• Accession No. Swiss-Prot: P24864
  NCBI Protein: NP_001229.1
• UniprotP24864
• Gene ID898;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 48kd
  Western blotting: 1:1000
  

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition.

Won K.A., Reed S.I.EMBO J. 15:4182-4193(1996)
Welcker M., Singer J., Loeb K.R., Grim J., Bloecher A., Mol. Cell 12:381-392(2003)

• Western blot analysis of extracts from 293 cells untreated or treated with hydroxyurea using Cyclin E1(phospho-Thr395) Antibody #11541.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting