• Product NameTF2E2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total TF2E2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human TF2E2.
• Target NameTF2E2
• Alternative Names    GTF2E2; T2EB; TF2E2; Transcription initiation factor IIE beta subunit;
• Accession No. Swiss-Prot: P29084
  NCBI Gene ID: 2961
• UniprotP29084
• Gene ID2961;
• SDS-PAGE MW 33kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.

Peterson M.G., Nature 354:369-373(1991).
Sumimoto H., Nature 354:401-404(1991).
Kang S.-W., Genes Cells 5:251-263(2000).

• Western blot analysis of extracts from Jurkat cells, using TF2E2 antibody #33739.
• Immunohistochemistry analysis of paraffin-embedded human heart tissue using TF2E2 antibody #33739.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting