• Product NameELF1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total ELF1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human ELF1.
• Target NameELF1
• Alternative Names    E74-like factor 1; ELF1; ETS-related transcription factor Elf-1;
• Accession No. Swiss-Prot: P32519
  NCBI Gene ID: 1997
• UniprotP32519
• Gene ID1997;
• SDS-PAGE MW 67kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.

Leiden J.M., J. Virol. 66:5890-5897(1992).
Thompson C.B., Mol. Cell. Biol. 12:1043-1053(1992).
Wang C.Y., Science 260:1330-1335(1993).

• Western blot analysis of extracts from 293 cells and HuvEc cells, using ELF1 antibody #33750.
• Immunofluorescence analysis of A549 cells, using ELF1 antibody #33750.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting