• Product NameCKI-α Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total CKI-α protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CKI-α.
• Target NameCKI-α
• Alternative Names    CK1-alpha; CKI-alpha; CSNK1A1; Casein kinase I; alpha isoform
• Accession No. Swiss-Prot: P48729
  NCBI Gene ID: 1452
• UniprotP48729
• Gene ID1452;
• SDS-PAGE MW 35kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis.

Tapia C., FEBS Lett. 349:307-312(1994).
Fish K.J., J. Biol. Chem. 270:14875-14883(1995).
Liu C., Cell 108:837-847(2002).

• Western blot analysis of extracts from HeLa cells, using CKI-α antibody #33780.
• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using CKI-α antibody #33780.
• Immunofluorescence analysis of HeLa cells, using CKI-α antibody #33780.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting