• Product NameCLK1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total CLK1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CLK1.
• Target NameCLK1
• Alternative Names    CDC like kinase 1; CLK; CLK1; Dual specificity protein kinase CLK1; EC 2.7.1.112
• Accession No. Swiss-Prot: P49759
  NCBI Gene ID: 1195
• UniprotP49759
• Gene ID1195;
• SDS-PAGE MW 57kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates: SRSF1, SRSF3 and PTPN1. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells and adenovirus E1A pre-mRNA.

Johnson K.W., J. Biol. Chem. 266:3402-3407(1991).
Hanes J.J., J. Mol. Biol. 244:665-672(1994).
Olsen J.V., Cell 127:635-648(2006).

• Western blot analysis of extracts from 293 cells, using CLK1 antibody #33784.
• Immunofluorescence analysis of HUVEC cells, using CLK1 antibody #33784.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting