• Product NameLIMK2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total LIMK2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human LIMK2.
• Target NameLIMK2
• Alternative Names    EC 2.7.11.1; LIM domain kinase 2; LIMK-2; kinase LIMK2;
• Accession No. Swiss-Prot: P53671
  NCBI Gene ID: 3985
• UniprotP53671
• Gene ID3985;
• SDS-PAGE MW 70kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.

Okano I., J. Biol. Chem. 270:31321-31330(1995).
Osada H., Biochem. Biophys. Res. Commun. 229:582-589(1996).
Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).

• Western blot analysis of extracts from HepG2 cells and HUVEC cells, using LIMK2 antibody #33815.
• Immunohistochemistry analysis of paraffin-embedded human heart tissue using LIMK2 antibody #33815.
• Immunofluorescence analysis of HeLa cells, using LIMK2 antibody #33815.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting