• Product NameELL Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total ELL protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human ELL.
• Target NameELL
• Alternative Names    RNA polymerase II elongation factor ELL; RNA polymerase II elongation factor ELL; ELL; C19orf17;
• Accession No. Swiss-Prot: P55199
  NCBI Gene ID: 8178
• UniprotP55199
• Gene ID8178;
• SDS-PAGE MW 65kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation.

Thirman M.J., Proc. Natl. Acad. Sci. U.S.A. 91:12110-12114(1994).
Simone F., Blood 98:201-209(2001).
Kong S.E., Proc. Natl. Acad. Sci. U.S.A. 102:10094-10098(2005).

• Western blot analysis of extracts from Jurkat cells and HeLa cells, using ELL antibody #33821.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting