• Product NameDLX5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total DLX5 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human DLX5.
• Target NameDLX5
• Alternative Names    Homeobox protein DLX-5; DLX5;
• Accession No. Swiss-Prot: P56178
  NCBI Gene ID: 1749
• UniprotP56178
• Gene ID1749;
• SDS-PAGE MW 32kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Transcriptional factor involved in bone development. Acts as an immediate early BMP-responsive transcriptional activator essential for osteoblast differentiation. Stimulates ALPL promoter activity in a RUNX2-independent manner during osteoblast differentiation. Stimulates SP7 promoter activity during osteoblast differentiation. Promotes cell proliferation by up-regulating MYC promoter activity. Involved as a positive regulator of both chondrogenesis and chondrocyte hypertrophy in the endochondral skeleton. Binds to the homeodomain-response element of the ALPL and SP7 promoter. Binds to the MYC promoter. Requires the 5'-TAATTA-3' consensus sequence for DNA-binding.

Ota T., Nat. Genet. 36:40-45(2004).
Hillier L.W., Nature 424:157-164(2003).
Willis D.M., J. Biol. Chem. 277:37280-37291(2002).

• Western blot analysis of extracts from HeLa cells, 293 cells and COLO205 cells, using DLX5 antibody #33825.
• Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using DLX5 antibody #33825.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting