• Product NameTNFC Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total TNFC protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human TNFC.
• Target NameTNFC
• Alternative Names    Lymphotoxin-beta; LT-beta; Tumor necrosis factor C; TNF-C; Tumor necrosis factor ligand superfamily member 3
• Accession No. Swiss-Prot: Q06643
  NCBI Gene ID: 4050
• UniprotQ06643
• Gene ID4050;
• SDS-PAGE MW 22kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  Immunofluorescence: 1:100~1:500

Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface. Isoform 2 is probably non-functional.

Browning J.L., Cell 72:847-856(1993).
Warzocha K., Biochem. Biophys. Res. Commun. 238:273-276(1997).
Neville M.J., J. Immunol. 162:4745-4754(1999).

• Western blot analysis of extracts from HepG2 cells, using TNFC antibody #33861.
• Immunofluorescence analysis of HeLa cells, using TNFC antibody #33861.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting