• Product NameTF2H2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total TF2H2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human TF2H2.
• Target NameTF2H2
• Alternative Names    TFIIH basal transcription factor complex p44 subunit; Basic transcription factor 2 44 kDa subunit; BTF2-p44; General transcription factor IIH polypeptide 2; GTF2H2
• Accession No. Swiss-Prot: Q13888
  NCBI Gene ID: 2966
• UniprotQ13888
• Gene ID2966;
• SDS-PAGE MW 62kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. The N-terminus interacts with and regulates XPD whereas an intact C-terminus is required for a successful escape of RNAP II form the promoter.

Humbert S., EMBO J. 13:2393-2398(1994).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
van der Steege G., Eur. J. Hum. Genet. 3:87-95(1995).

• Western blot analysis of extracts from COLO205 cells, using TF2H2 antibody #33874.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting