• Product NameCBF β Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total CBF β protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CBF β.
• Target NameCBF β
• Alternative Names    Core-binding factor subunit beta; CBF-beta; Polyomavirus enhancer-binding protein 2 beta subunit; PEBP2-beta; PEA2-beta
• Accession No. Swiss-Prot: Q13951
  NCBI Gene ID: 865
• UniprotQ13951
• Gene ID865;
• SDS-PAGE MW 22kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBFB enhances DNA binding by RUNX1.

Hajra A., Genomics 26:571-579(1995).
Liu P., Science 261:1041-1044(1993).
Warren A.J., EMBO J. 19:3004-3015(2000).

• Western blot analysis of extracts from HeLa cells and HUVEC cells, using CBF β antibody #33875.
• Immunohistochemistry analysis of paraffin-embedded human heart tissue using CBF β antibody #33875.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting