• Product NameE2F2 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total E2F2 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human E2F2.
• Target NameE2F2
• Alternative Names    Transcription factor E2F2; E2F-2;
• Accession No. Swiss-Prot: Q14209
  NCBI Gene ID: 1870
• UniprotQ14209
• Gene ID1870;
• SDS-PAGE MW 48kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.

Ivey-Hoyle M., Mol. Cell. Biol. 13:7802-7812(1993).
Gregory S.G., Nature 441:315-321(2006).
Novy M., Mol. Biol. Cell 16:2181-2190(2005).

• Western blot analysis of extracts from HepG2 cells, K562 cells and COLO205 cells, using E2F2 antibody #33877.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting