• Product NameNR1I3 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total NR1I3 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human NR1I3.
• Target NameNR1I3
• Alternative Names    CAR; Constitutive androstane receptor; Orphan nuclear receptor MB67; Orphan nuclear receptor NR1I3;
• Accession No. Swiss-Prot: Q14994
  NCBI Gene ID: 9970
• UniprotQ14994
• Gene ID9970;
• SDS-PAGE MW 35kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element.

Baes M., Mol. Cell. Biol. 14:1544-1552(1994).
Choi H.S., J. Steroid Biochem. Mol. Biol. 56:23-30(1996).
Xu R.X., Mol. Cell 16:919-928(2004).

• Western blot analysis of extracts from Jurkat cells, treated with serum (20%, 15mins), using NR1I3 antibody #33887.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting