• Product NameMED1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total MED1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MED1.
• Target NameMED1
• Alternative Names    Mediator complex subunit 1;
• Accession No. Swiss-Prot: Q15648
  NCBI Gene ID: 5469
• UniprotQ15648
• Gene ID5469;
• SDS-PAGE MW 170kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Drane P., Oncogene 15:3013-3024(1997).
Yuan C.-X., Proc. Natl. Acad. Sci. U.S.A. 95:7939-7944(1998).
Naeaer A.M., Nature 398:828-832(1999).

• Western blot analysis of extracts from Jurkat cells, using MED1 antibody #33902.
• Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using MED1 antibody #33902.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting