• Product NameDOK7 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total DOK7 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human DOK7.
• Target NameDOK7
• Alternative Names    Dok-7; downstream of tyrosine kinase 7;
• Accession No. Swiss-Prot: Q18PE1
  NCBI Gene ID: 285489
• UniprotQ18PE1
• Gene ID285489;
• SDS-PAGE MW 60kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Probable muscle-intrinsic activator of MUSK that plays an essential role in neuromuscular synaptogenesis. Acts in aneural activation of MUSK and subsequent acetylcholine receptor (AchR) clustering in myotubes. Induces autophosphorylation of MUSK.

Okada K., Science 312:1802-1805(2006).
Ota T., Nat. Genet. 36:40-45(2004).
Beeson D., Science 313:1975-1978(2006).

• Western blot analysis of extracts from mouse brain cells, using DOK7 antibody #33918.
• Immunohistochemistry analysis of paraffin-embedded human brain tissue using DOK7 antibody #33918.
• Immunofluorescence analysis of HepG2 cells, using DOK7 antibody #33918.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting