• Product NameCERKL Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total CERKL protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human CERKL.
• Target NameCERKL
• Alternative Names    Ceramide kinase-like protein; CERKL;
• Accession No. Swiss-Prot: Q49MI3
  NCBI Gene ID: 375298
• UniprotQ49MI3
• Gene ID375298;
• SDS-PAGE MW 63kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Has no detectable ceramide-kinase activity. Overexpression of CERKL protects cells from apoptosis in oxidative stress conditions.

Tuson M., Am. J. Hum. Genet. 74:128-138(2004).
Bornancin F., Biochim. Biophys. Acta 1687:31-43(2005).
Ota T., Nat. Genet. 36:40-45(2004).

• Western blot analysis of extracts from A549 cells, using CERKL antibody #33922.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting