• Product NameMAP2K4 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous levels of total MAP2K4 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.352~356(E-S-K-R-P)derived from Human MAP2K4.
• Target NameMAP2K4
• Alternative Names    JNKK1; MEK4; MKK4; PRKMK4; SERK1
• Accession No. Swiss-Prot: P45985
  NCBI Protein: NP_003001.1
• UniprotP45985
• Gene ID6416;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 44kd
  Western blotting: 1:500~1:1000
  

SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).

Davis, R.J. (1994) Trends Biochem. Sci. 19, 470-473.
Sanchez, I. et al. (1994) Nature 372, 794-798.
Yan, M. et al. (1994) Nature 372, 798-800.
Kyriakis, J.M. et al. (1994) Nature 369, 156-160.
Park, H. et al. (2002) J. Biol. Chem. 277, 2573-2578.

• Western blot analysis of extracts from HUVEC cells using MAP2K4 Antibody #21441.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting