• Product NameDGKH Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsIHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total DGKH protein
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human DGKH.
• Target NameDGKH
• Alternative Names    iacylglycerol kinase eta; EC 2.7.1.107; Diglyceride kinase eta; DGK-eta; DAG kinase eta
• Accession No. Swiss-Prot: Q86XP1
  NCBI Gene ID: 160851
• UniprotQ86XP1
• Gene ID160851;
• SDS-PAGE MW 135kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Plays a key role in promoting cell growth. Activates the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by EGF. Regulates the recruitment of RAF1 and BRAF from cytoplasm to membranes and their heterodimerization.

Murakami T., J. Biol. Chem. 278:34364-34372(2003) .
Dunham A., Nature 428:522-528(2004).
Ota T., Nat. Genet. 36:40-45(2004).

• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using DGKH antibody #33954.
• Immunofluorescence analysis of HeLa cells, using DGKH antibody #33954.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting