• Product NamePPHLN Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total PPHLN protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human PPHLN.
• Target NamePPHLN
• Alternative Names    Periphilin-1; Gastric cancer antigen Ga50; PPHLN1; HSPC206; HSPC232
• Accession No. Swiss-Prot: Q8NEY8
  NCBI Gene ID: 51535
• UniprotQ8NEY8
• Gene ID51535;
• SDS-PAGE MW 53kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation.

Line A., Br. J. Cancer 86:1824-1830(2002).
Kazerounian S., J. Biol. Chem. 278:36707-36717(2003).
Zhang Q.-H., Genome Res. 10:1546-1560(2000).

• Western blot analysis of extracts from COLO cells and 293 cells, using PPHLN antibody #33975.
• Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using PPHLN antibody #33975.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting