• Product NameMEKKK 1 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF ELISA
• Species ReactivityHuman Mouse
• SpecificityThe antibody detects endogenous levels of total MEKKK 1 protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human MEKKK 1.
• Target NameMEKKK 1
• Alternative Names    Mitogen-activated protein kinase kinase kinase kinase 1; EC 2.7.11.1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1
• Accession No. Swiss-Prot: Q92918
  NCBI Gene ID: 11184
• UniprotQ92918
• Gene ID11184;
• SDS-PAGE MW 91kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• WB 1:500-1:2000
  IF 1:50-200
  ELISA 1:10000;

May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.

Hu M.C.-T., Genes Dev. 10:2251-2264(1996).
Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
Wissing J., Mol. Cell. Proteomics 6:537-547(2007).

• Western blot analysis of extracts from HepG2 cells, using MEKKK 1 antibody #34009.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting