• Product NameHistone H2B Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total Histone H2B protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from internal of human Histone H2B.
• Target NameHistone H2B
• Alternative Names    Histone H2B type 1-H; H2B.j; H2B/j; HIST1H2BH; H2BFJ
• Accession No. Swiss-Prot: Q93079
  NCBI Gene ID: 8345
• UniprotQ93079
• Gene ID8345;
• SDS-PAGE MW 18kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  Immunofluorescence: 1:100~1:500

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Albig W., Genomics 40:314-322(1997).
Marzluff W.F., Genomics 80:487-498(2002).
Mungall A.J., Nature 425:805-811(2003).

• Western blot analysis of extracts from A549 cells, using Histone H2B antibody #34015.
• Immunohistochemistry analysis of paraffin-embedded human brain tissue using Histone H2B antibody #34015.
• Immunofluorescence analysis of HeLa cells, using Histone H2B antibody #34015.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting