• Product NameHES6 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous levels of total HES6.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from N-terminal of human HES6.
• Target NameHES6
• Alternative Names    Transcription cofactor HES-6; Hairy and enhancer of split 6; C-HAIRY1; HES6;
• Accession No. Swiss-Prot: Q96HZ4
  NCBI Gene ID: 55502
• UniprotQ96HZ4
• Gene ID55502;
• SDS-PAGE MW 30kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  
  

Does not bind DNA itself but suppresses both HES1-mediated N box-dependent transcriptional repression and binding of HES1 to E box sequences. Also suppresses HES1-mediated inhibition of the heterodimer formed by ASCL1/MASH1 and TCF3/E47, allowing ASCL1 and TCF3 to up-regulate transcription in its presence. Promotes cell differentiation By similarity. UniProtKB Q9JHE6

Bae S.-K., Development 127:2933-2943(2000).
Vasiliauskas D., Mech. Dev. 98:133-137(2000).
Ota T., Nat. Genet. 36:40-45(2004).

• Western blot analysis of extracts from HeLa cells, using HES6 antibody #34030.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting