• Product NameMEK5 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total MEK5 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa. 311-315(K-T-Y-V-G ) derived from Rat MEK5.
• Target NameMEK5
• Alternative Names    MAP kinase kinase 5; Map2k5; MAPKK 5; MAPK/ERK kinase 5;
• Accession No. Swiss-Prot: Q62862
  NCBI Protein: NP_001029159.1
• UniprotQ62862
• Gene ID29568;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 49kd
  Western blotting: 1:500
  

A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phospho-rylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resem-bles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphory-late ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

Han, J., et al. 1996. J. Biol. Chem. 271: 2886-2891.
Jiang, Y., et al. 1996. J. Biol. Chem. 271: 17920-17926.
Tournier, C., et al. 1997. Proc. Natl. Acad. Sci. USA94: 7337-7442.
Holland, P.M., et al. 1997. J. Biol. Chem. 272: 24994-24998.

• Western blot analysis of extract from rat brain and mouse brain tissue using MEK5 Antibody #21559

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting