• Product NameCD44 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB IF
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total CD44 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.734~738(Q-N-V-D-M) derived from Human CD44.
• Target NameCD44
• Alternative Names    LHR; MDU2; MDU3; MIC4;
• Accession No. Swiss-Prot: P16070
  NCBI Protein: NP_000601.3
• UniprotP16070
• Gene ID960;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 80kd
  Western blotting: 1:500~1:1000
  Immunofluorescence:1:100~1:200

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.

Teriete P.,et.al(2004) Mol. Cell 13:483-496 Bartolazzi A. (2003)Melanoma Res. 13:325-337
Liu T.et.al (2005)J. Proteome Res. 4:2070-2080

• Western blot analysis of extract from Hela cells using CD44 Antibody #21471
• Immunofluorescence staining of methanol-fixed NCI-h446 cells showing cytoplasmic staining using CD44 Antibody #21471.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting