• Product NameDUS2L Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• ApplicationsWB IHC
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous levels of total DUS2L protein.
• Immunogen TypePeptide
• Immunogen DescriptionSynthesized peptide derived from C-terminal of human DUS2L.
• Target NameDUS2L
• Alternative Names    tRNA-dihydrouridine synthase 2-like; EC 1.-.-.-; hDUS2; Up-regulated in lung cancer; URLC8
• Accession No. Swiss-Prot: Q9NX74
  NCBI Gene ID: 54920
• UniprotQ9NX74
• Gene ID54920;
• SDS-PAGE MW 55kd
• Concentration 1.0mg/ml
• Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20˚C

• Western blotting: 1:500~1:3000
  Immunohistochemistry: 1:50~1:100
  

Dihydrouridine synthase. Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Negatively regulates the activation of EIF2AK2/PKR.

Ota T., Nat. Genet. 36:40-45(2004).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Kato T., Cancer Res. 65:5638-5646(2005).

• Western blot analysis of extracts from RAW264.7 cells, using DUS2L antibody #34081.
• Immunohistochemistry analysis of paraffin-embedded human skeletal muscle using DUS2L antibody #34081.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting