• Product NameE-Cadherin(CD324) Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total E-Cadherin protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.864~868(E-W-G-N-R) derived from Human E-Cadherin.
• Target NameE-Cadherin(CD324)
• Alternative Names    CDH1; CDHE; UVO
• Accession No. Swiss-Prot: P12830
  NCBI Protein: NP_004351.1
• UniprotP12830
• Gene ID999;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 135kd
  Western blotting: 1:500~1:1000
  

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin a-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Baki L.et.al(2001)Proc. Natl. Acad. Sci. U.S.A. 98:2381-2386
Makagiansar I.T.et.al(2002)J. Biol. Chem. 277:16002-16010
Agiostratidou G. (2006)J. Neurochem. 96:1182-1188

• Western blot analysis of extract from MCF-7 and HT29 cells using E-Cadherin(CD324) Antibody #21473

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting