• Product NameREST Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total REST protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.873~877(R-E-E-A-S) derived from Human REST.
• Target NameREST
• Alternative Names    NRSF; XBR; REST
• Accession No. Swiss-Prot: Q13127
  NCBI Protein: NP_001180437.1
• UniprotQ13127
• Gene ID5978;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 121kd
  Western blotting: 1:500~1:1000
  

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.

Chong J.A.et.al. (1995)Cell 80:949-957
Schoenherr C.J.et.al.(1995)Science 267:1360-1363
Scholl T.et.al.(1996)J. Immunol. 156:1448-1457
Lunyak V.V.et.al. (2002)Science 298:1747-1752

• Western blot analysis of extract from Hela cells using REST Antibody #21475

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting