• Product NameNSE Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total NSE protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.6~10(I-W-A-R-E)derived from Human NSE.
• Target NameNSE
• Alternative Names    ENO2
• Accession No. Swiss-Prot: P09104
  NCBI Protein: NP_001966.1
• UniprotP09104
• Gene ID2026;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 47kd
  Western blotting: 1:500~1:1000
  

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

McAleese S.M., Dunbar B., Fothergill J., Hinks L., Day I.N.M.Eur. J. Biochem. 178:413-417(1988)

• Western blot analysis of extract from JK, HUVEC, HepG2 and Hela cells using NSE Antibody #21487

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting