• Product NameCD10 Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu Ms Rt
• SpecificityThe antibody detects endogenous level of total CD10 protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.6~10(S-Q-M-D-I) derived from Human CD10.
• Target NameCD10
• Alternative Names    NEP; MME; SFE; CALLA; MGC126681
• Accession No. Swiss-Prot: P08473
  NCBI Protein: NP_000893.2
• UniprotP08473
• Gene ID4311;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 100kd
  Western blotting: 1:500
  

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.

Yandle T.G., Brennan S.O., Espiner E.A., Nicholls M.G., Richards A.M.Peptides 10:891-894(1989)
Rice G.I., Thomas D.A., Grant P.J., Turner A.J., Hooper N.M.Biochem. J. 383:45-51(2004)
Morisaki N., Moriwaki S., Sugiyama-Nakagiri Y., Haketa K., Takema Y., Imokawa G.J. Biol. Chem. 285:39819-39827(2010)

• Western blot analysis of extract from Rat kidney tissue using CD10 Antibody #21490

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting