• Product NameERAB Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total ERAB protein.
• Immunogen TypePeptide-KLH
• Immunogen DescriptionPeptide sequence around aa.253~257(L-D-G-A-I)derived from Human ERAB.
• Target NameERAB
• Alternative Names    ABAD; CAMR; HCD2; MHBD; HADH2
• Accession No. Swiss-Prot: Q99714
  NCBI Protein: NP_004484.1
• UniprotQ99714
• Gene ID3028;
• Concentration 1.0mg/ml
• Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

• Predicted MW: 27kd
  Western blotting: 1:500~1:1000
  

Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/RG9MTD1, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. By interacting with intracellular amyloid-beta, it may contribute to the neuronal dysfunction associated with Alzheimer disease (AD).

Holzmann J., Frank P., Loeffler E., Bennett K.L., Gerner C., Rossmanith W. Cell 135:462-474(2008)

• Western blot analysis of extract from JK, HUVEC, HepG2 and Hela cells using ERAB Antibody #21602

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting